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Figure 1: Effect of 15d-PGJ2 on surface molecules expression. BMDC were subjected to the pretreatment with 15d-PGJ2(1, 5, and 10μM), for 1 hour, and then stimulated with LPS. After 24 hours of stimulation, the BMDC were harvested and double-stained for CD11c or CD80, CD86, and MHC-II. Monoclonal antibody conjugated PE or FITC was used for staining and was detected through flow cytometry.