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Figure 2: A) Schematic representation of the compartments of the nucleus in interphase NE: nuclear envelope, NPC: nuclear pore complex, NL: nuclear lamina, NM: nuclear matrix, CTs: chromosome territories, MARs: matrix attachment regions that correspond to DNA sequences that allow the interaction of chromatin to NM, and LADs: lamin attachment domains, corresponding to DNA sequences that allow the interaction of chromatin to the nuclear lamina. B-C) 3T3-L1 preadipocytes grown on coverslips were incubated with DEXA in the absence (B) or presence (C) of IBMX and subjected to indirect immunofluorescence with anti-GR, anti-FKBP51, and secondary antibodies labeled with Alexa 488 or Alexa 546, respectively, and images were analyzed by confocal microscopy. GR is detected in red, FKBP51 is detected in green, and chromatin is stained by DAPI in blue. Inserts in each panel were magnified. Observed in panel C is that DEXA plus IBMX treatment caused enrichment of GR (red signal) in the nuclear area limited by the white dotted lines that correspond to NL.