Figure 2: PR expression in U373 human astrocytoma cells. Immunofluorescence images were quantified and analyzed as described in Materials and Methods. Vehicle (V); 48 h with estradiol (E) (10 nM); E followed by progesterone (P) (10 nM) for 24 h (E+P); E followed by RU486 (1 µM) for 24 h (E+RU); E followed by P+RU486 for 24 h (E+P+RU). Results are expressed as mean ± SEM; n=5. **P<0.05 vs all groups; *P<0.05 vs the other groups except V in cytoplasm.