Figure 1: LXR activation inhibits proliferation and reduces Akt phosphorylation.(A) MCF7, SW480, and HUH7 cells were treated with vehicle (DMSO) or 5 μM GW3965 for 48 h. Cells were harvested, a viability test was performed, and the absolute number of viable cells was counted. MCF7 cells were cultured in 1% serum medium and then treated with DMSO and 5 μM GW3965 for 16 h. Cells were harvested either for the viability assay test to count absolute number of viable cells after activating the cells with IGF-1 (50ng/ml) in different time points (B) or for immunoblotting after activating them with (50ng/ml) IGF-1 (20 min) (C). (D) MCF7, SW480, and HUH7 cells were cultured with 1% serum in growth medium and treated with 5 μM GW3965 for 16 h. Cells were given IGF-1 (50ng/ml) for 20 min, whole cell extract isolated and Western immunoblotting performed with various antibodies as described in the figure. GAPDH was used as an internal control demonstrating equal protein loading. The data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and #P < 0.0001 versus vehicle by Student's t-test.