Figure 5: LXRβ mediates the antiproliferative effect of LXR.(A) The efficacy of silencing LXRs was analyzed under the condition mentioned in (B and C). (B) MDA-MB-231 cells were transduced with LXRα and LXRβ shRNA lentivirus and (C) MCF7 cells were transfected with 10 nM siLXRβ, for 48 h, and treated with DMSO or 5 μM GW3965 for 16 h. Cells were given IGF-1 (50ng/ml) for 20 min, whole cell extract isolated and Western immunoblotting performed. GAPDH was used as an internal control demonstrating equal protein loading. The relative level of phosphorylation was quantified as described in material and methods. (D) MCF7 cells were treated with 5 μM GW3965 for 48 h, cells were harvested, a viability test was performed, and the absolute number of viable cells was counted. The data are presented as mean ± SEM. **P < 0.01 and #P < 0.0001 versus vehicle by Student's t-test.