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Figure 4: Monitorization of the effect of different agonists on [Ca2+]c in freshly prepared pancreatic acinar cells. Cells were isolated as described in material and methods section, and then checked at day 0 (i.e., cells were not subjected to culture) for Ca2+ responses after stimulation with physiological agonists. (A) Time-course of changes in [Ca2+]c after stimulation with 1 nM CCK-8. (B) Ca2+ response observed after stimulation of cells with 100 nM ACh. (C) Typical oscillatory pattern of Ca2+ mobilization in response to stimulation of freshly prepared pancreatic acinar cells with 20 pM CCK-8. The experiments were performedin the presence of Ca2+ in the extracellular medium. Viability of cells was not decreased under culture conditions and in the absence of stimulus. Stimuli were added to the cells on the day in which the tests for Ca2+ mobilization were performed. The horizontal bar indicates the time during which the stimulus was applied to the cells. Traces are representative of 48–71 cells analyzed in 5 independent experiments for each treatment.