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Research Article
The Open Access Journal of Science and Technology
Vol. 2 (2014), Article ID 101061, 9 pages
doi:10.11131/2014/101061

Analysis of The Progress of Enzyme-Catalyzed Reaction

Alfonsas Juška

Vilniaus Gedimino technikos universitetas, Saulëtekio al. 11, 10223 Vilnius, Onkologijos institutas, Vilniaus universitetas, Baublio g. 3b, LT-08406 Vilnius, Lithuania

Received 18 December 2013; Accepted 20 July 2014

Academic Editor: Yusuf Tutar

Copyright © 2014 Alfonsas Juška. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the research is to analyze and model the progress of product accumulation in relation with the kinetic properties of the enzyme and substrate concentration. Conventional methods of analysis are used. The results are as follows: `linear' decline of high substrate concentration (or rise of the product) is determined by the kinetic properties of the enzyme taking into account initial substrate concentration (Model 1); the exponential decline corresponds to Model 2; the deviations from linearity occurring either because of product inhibition of the reaction, the backward reaction or substrate exhaustion are modelled by algebraic sum of linear and exponential functions. Modification of the conventional scheme of the enzyme-catalyzed reaction, therefore, facilitates deriving the equations highlighting the role of the reaction product and initial substrate concentration in the progress of the reaction. The equations enable estimation of the kinetic parameters of the enzyme from the data of conventional experiments and an additional experiment corresponding to the `end' of the reaction.

Research Article
The Open Access Journal of Science and Technology
Vol. 2 (2014), Article ID 101061, 9 pages
doi:10.11131/2014/101061

Analysis of The Progress of Enzyme-Catalyzed Reaction

Alfonsas Juška

Vilniaus Gedimino technikos universitetas, Saulëtekio al. 11, 10223 Vilnius, Onkologijos institutas, Vilniaus universitetas, Baublio g. 3b, LT-08406 Vilnius, Lithuania

Received 18 December 2013; Accepted 20 July 2014

Academic Editor: Yusuf Tutar

Copyright © 2014 Alfonsas Juška. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the research is to analyze and model the progress of product accumulation in relation with the kinetic properties of the enzyme and substrate concentration. Conventional methods of analysis are used. The results are as follows: `linear' decline of high substrate concentration (or rise of the product) is determined by the kinetic properties of the enzyme taking into account initial substrate concentration (Model 1); the exponential decline corresponds to Model 2; the deviations from linearity occurring either because of product inhibition of the reaction, the backward reaction or substrate exhaustion are modelled by algebraic sum of linear and exponential functions. Modification of the conventional scheme of the enzyme-catalyzed reaction, therefore, facilitates deriving the equations highlighting the role of the reaction product and initial substrate concentration in the progress of the reaction. The equations enable estimation of the kinetic parameters of the enzyme from the data of conventional experiments and an additional experiment corresponding to the `end' of the reaction.