|Figure 2: Flow cytometry analysis of expanded T-lymphocytes.
(a) Flow cytometry diagram illustrating significant increase in both CD8 and CD4 T
lymphocytes from both control and dendritised culture flask. Beyond Day 28 of lymphoexpansion,
there was a greater increase in CD4 T lymphocytes in comparison to CD8 T lymphocytes
for both control and dendritised culture flask. (b) Both CD4 positive T lymphocytes
from control and dendritised flasks exhibit effector memory (TEM) phenotype (CD45RA−,
CD45RO+, CD62L−, CCR7−, CD127−, CD25+, and CD27±, CD28±). (c) There was a significantly
higher expression of INF-γ in CD4 positive T lymphocytes that was co-cultured with
dendritised leukemic blast cells in comparison to the control CD4 positive T lymphocytes.
There was also a slightly higher amount of granzyme A and granulysin in T lymphocytes
that was co-cultured with “dendritised” leukemic blast cells than the control CD4
positive T lymphocytes. Unfilled Histogram = Control CD 4 T- Lymphocytes
Filled Histogram = CD4 T- Lymphocytes Co-Cultured with “dendritised” Blast Cells.