|Figure 3: Cytotoxicity Assay profile of CD4 T lymphocytes from dendritised blast cells and control.
(a) Flow cytometry analysis of leukemic blast cells that are CD117 positive. (i) Flow
shows significant decrease in the population of 7AAD and Annexin V double negative
leukemic blast cells with increasing ratio of effector cells: target cells. (ii) Flow
cytometry diagram shows relatively constant population of 7AAD and Annexin V double
negative leukemic blast cells throughout all ratios of effector cells: target cells.
Figure 3(a) is illustrative of patient 2.
(b) Cumulative average percentage of dead leukemic blast cells for effector:target
(0:1, 1:1, 2.5:1, 10:1). There was a superiority of cytotoxicity for CD4 T lymphocytes
from dendritised blast cells in comparison to control CD4 T lymphocytes. At effector:target
ratio of 10:1, the CD4 T lymphocytes that were primed from “dendritised” blast cells
exhibited close to a significant 15% cytotoxicity. Values are obtained from the cumulative
average of patient 1–3 in this study, with two set of values for patient 1.