Author(s): Jiri Hatina
Soft tissue sarcomas are known for their great variability in clinical behavior, ranging from almost indolent lesions to rapidly metastasing tumours. Genes responsible for sarcoma progression have been poorly characterized by now. Towards this end, we comprehensively analyzed transcriptomes of two single-background progression series of murine sarcoma cell lines. The first one, established by us from consecutive fibrosarcomas in a v-jun transgenic mouse, consisted of the slowly proliferating nonmotile and noninvasive cell line JUN-2, rapidly proliferating, motile and invasive cell line JUN-3, and the cell line JUN-2fos-3 that exhibits a unique transformation pattern, with little deregulation of cell growth and proliferation, but pronounced motility and invasiveness.
The second one, established by a french group, consisted of the widely used pre-adipocytic cell line 3T3L1 and its derivative liposarcoma cell line LM3D. From both liposarcoma cell lines, we isolated putative liposarcoma stem cells (as side-population cells).
We performed four sets of genome-wide transcriptomic analyses. The JUN-fibrosarcoma progression series, by virtue of its unique distribution of transformation related-traits between individual cell lines, made us possible to identify two separate groups of genes tentatively involved in sarcoma progression in a single transcriptomic analysis on the one hand, proliferation-related genes could be identified by their differential expression in JUN-3 compared to both JUN-2 and JUN-2fos3, and, on the other hand, motility and invasiveness-related genes could be identified by their common expression pattern in JUN-2fos3 and JUN-3 cells compared to JUN-2.
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