Figure 5: Monitorization of the effect of different agonists on [Ca2+]c in pancreatic acinar cells cultured for 48 hours. Pancreatic acinar cells were allowed to grow for 48 hours. Then cells were loaded with fura-2 and fluorescence was analyzed after stimulation of cells with 1 nM CCK-8 (A), 100 nM ACh (B) or 20 pM CCK-8 (C). The changes in [Ca2+]c did not follow the typical response pattern shown by pancreatic acinar cells at day zero of culture. Viability of cells was not decreased under culture conditions and in the absence of stimulus. Stimuli were added to the cells on the day in which the tests for Ca2+ mobilization were performed. Traces are representative of 22–35 cells analyzed in 5-6 independent experiments for each treatment.