Figure 7: Analysis of viability of pancreatic acinar cells in culture. Cell viability under the different treatments applied was analyzed studying AlamarBlue reduction, as described in “Material and methods”. (A) Incubation of cells in the presence of 1 nM CCK-8 did not alter cell viability significantly along the incubation period. (B) Effect of incubation of cells with 1 μM Tps on cell viability. (C) H2O2 (100 μM) led to a decrease in cell viability compared to non-stimulated cells. The horizontal dotted line represents the viability of non-stimulated, which was considered 100%. Graphics are representative of 4 independent experiments for each treatment (*, P< 0.001 for treatments vs non-stimulated cells).