|Figure 1: Electrophoretic resolution of PCR products. A8 and E2 primers were employed against
the genomic DNA of newly isolated A. nicotianae strain PR with control DNA from bacterium
Exuguobacterium acetylicum strain SN under conditions described in materials and methods.
Lane 1 is DNA step ladder 100bp, lane 2: Exiguobacterium acetylicum strain SN and
lane 3: Arthrobacter nicotianae strain PR. Shown are amplicons ranging from 700 to
1000 base pairs in length. The amplified DNA of A. nicotianae was cloned in TA vectors
(Invitrogen) and sequenced. Significant matches of amplicon B were found in the NCBI-BLAST
searches which reveal its identity as Aldo-keto reductase (AKR).