Figure 1: Flow-cytometry analysis of co-stimulatory molecules (CD80 and CD86). MHC II and “dendritised” leukemic blast cells. (a) Distinctive increase in the co-stimulatory molecules (CD80 and CD86) and MHC II when compared between non-dendritised (Day 0) leukemic blast cells and dendritised blast cells at Day 7. Diagram shown is representative for Patient 1 and consistent with all 7 patients. Leukemic blast cells from 7 patients were co- cultured in a concortion of IL-4, GM-CSF and TNF-α over a period of 10 days. The dMFI of CD80, CD86 and MHC II peaked at approximately Day 7. (b) Flow cytometry analysis shows the progressive increase in the number of leukemic blast cells which are positive for stem cell marker CD34, expressing CD80, CD86 and MHC II as the dendritisation days increases from Day 0 to Day 7.