The Open Access Journal of Science and Technology

Abstract

In Vitro Generation of Cytotoxic CD4 Lymphocyte Response against Autologous Acute Myeloid Leukemia

Author(s): KokChong Yap,Liam Pock Ho,Pui San Yit,and Yeow Tee Goh

Relapsedandrefractorydiseaseshortensthesurvivalofacutemyeloidleukemia(AML)patients.Therefore,additional therapyapartfromconventionalchemotherapyandstemcelltransplantationisurgentlyneeded,especiallyineradicatingresidual leukemic stem cells. The identification of leukemia associated antigens, and the observation that administration of allogeneic T cellsmaymediategraftversusleukemiaeffectpavedthewaytodevelopvariousimmunotherapystrategies.Effectiveprovisionof bothtumorantigenandco-stimulationareessentialforstrategiesaimedatenrollingcytotoxicT-lymphocytestoeradicateleukemic cells.Inthisstudy,weattemptedtoup-regulateco-stimulatorsonleukemiccells(termed“dendritisation”tomimicdendriticcells inprovidingstrongco-stimulation)andcouplewithinherentleukemicantigenstogeneratespecificantileukemicT-lymphocytes againstautologousblastcells.OurstudyshowsevidenceofdistinctcytotoxicityofCD4positiveTlymphocytesagainstautologous blastcells.DendritisedAMLblastswereabletofunctionwithantigenpresentingcapability,whilestillphenotypicallyexistingas blastcells.ThiswasalsoachievableforawiderangeofsubtypesofAML,makingitapotentialimmunotherapyagainstAML

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